Effect of β-carotene on Cell Growth Inhibition of KB Human Oral Cancer Cells

β-carotene is present in carrots, pumpkins, and sweet potatoes. It suppresses many types of cancers by regulating cellular proliferation and apoptosis through a variety of mechanisms. However, the effects of β -carotene on oral cancer cells have not been clearly established. The main goal of this study was to investigate the effects of β-carotene on cell growth and apoptosis in oral cancer cells. Our results demonstrate that treatment with β-carotene induced inhibition of cell growth, and that the effect was dependent on β-carotene treatment time and concentration in KB cells. Furthermore, treatment with β-carotene induced nuclear condensation and fragmentation in KB cells. β-carotene promoted proteolytic cleavage of procaspase-3, -7, -8 and -9 with associated increases in the concentration of cleaved caspase-3, -7, -8 and -9. In addition, the level of cleaved PARP was increased by β-carotene treatment in KB cells. These results suggest that β-carotene can suppress cell growth and induce apoptosis in KB human oral cancer cells, and that it may have potential usefulness in anti-cancer drug discovery efforts.

Treatment of the Displaced Fractures of the Proximal Humerus by External fixation / 대한정형외과학회잡지

Treatment by External Fixation with External Compression Device was used for the displaced fractures of the proximal humerus. Especially in the old aged patient and patient with severe associated injury, this method was excellent for the early, painless motion of the shoulder and then good functional result. This experimental treatment was performed in 8 patients, and its merits were as follows: 1. The technique of operation was relatively easy, and was possible within a short time with minor injury. 2. The quality of reduction was good in all cases. 3. By the compression effect on the fracture site and firm maintenance of reduction during the treatment, solid healing as in internal fixation was possible. 4. Firm maintenance of reduction & minimal injury of soft tissue made possible early painless and wide ROM exercise, and then good functional result.

Experimental Treatment of the Patella Fractures by Modified Tension Band and External Fixator / 대한정형외과학회잡지

Experimental treatment by open reduction and internal fixation with modified tension band combined with external compression device was used for the patella fractures. Early post operative continuous passive motion and early weight bearing exercise was followed. This method was excellent for treatment of the patella fractures and prevention of post operative complications such as limitation of motion and post-traumatic arthritis of the knee joint. This experimental treatment was performed in 5 patients. and the results are as follows :1. Treatment by modified tension band combined with external fixator is useful for any type of the patella fracture. 2. By continuous passive motion and programed post operative rehabilitation, early painless recovery of wide range of motion of the knee joint and early weight bearing became possible. 3. Complications such as pain on motion, post-traumatic arthritis and limitation of motion of the knee joint was not seen. 4. This method is excellent for treatment of the patella fractures, and we are now trying to memodel the external fixator for cosmetic improvement and prevention of pin site problems.

Comparative Clinical Study Between Plating and Intramedullary Nailing of Femoral Shaft Fractures in Adult / 대한정형외과학회잡지

Various methods have been used in the treatment of femoral shaft fractures in adults. In recent years, generally accepted two methods are intramedullary nailing and plate fixation. We followed and reviewed 121 cases of femoral shaft fracture in 116 patients, treated by operation in Orthopaedic Department, Presbyterian Medical Center, Chonju from 1980 to 1986. All cases were devided into 2 groups, intramedullary nailing(24 cases) and plate fixation (97 cases), and the two groups were compared each other. The results are as follows : 1. Most of intramedullary nails were used in relatively younger age group, before 50 yesrs of age. 2. The mean bony union time in plate fixation was 14 weeks and that in intramedullary nailing was 17.4 weeks. They showed about 3 weeks of difference. 3. In the group, whose operations were performed within 2 weeks after injury, showed high rate of bone union within 6 months, but in those whose operations were delayed more than 2 weeks showed marked decrease in bone union. And the rate of decrease was greater in plate fixation. 4. The recovery of knee joint motion above 110 was greater in intramedullary nailing (87.5%) than that in plate fixation(79.3%). 5. There was no difference in functional recovery between plate fixation and intramedullary nailing. 6. The complications, including delayed and nonunion, infection, metal failure, and limitation of knee joint motion, were more in plate fixation. 7. The risk of refracture is higher in plate fixation. Therefore plates must be removed after some period.